Figure 2.
Effect of the androgen agonist Mes on H2S biosynthesis in isolated aortic rings. In a separate set of experiments aortic rings were incubated with PAG (10 mM, 15 min), then a cumulative concentration–response curve to Mes was performed (A). Relaxant effect of anabolic agent ST was also tested on isolated aortic rings (B). Statistical analysis was by two-way anova with a Bonferroni post hoc test (***P < 0.001 vs. T; °°°P < 0.001 vs. Mes; n = 6). Aortic tissues were incubated with Mes (10 μM) for 15 or 30 min, and H2S was determined as described in the Methods section (C). The same experimental protocol was followed using ST (10 μM) to stimulate H2S biosynthesis in aortic tissues (D). Statistical analysis was by one-way anova with Dunnett's post hoc test [##P < 0.01 vs. basal; °P < 0.05 and °°°P < 0.001 vs. L-cysteine (L-Cys); n = 6].