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. 2014 Jul 2;172(6):1505–1515. doi: 10.1111/bph.12740

Figure 4.

Figure 4

Interaction of NR3C4 (AR), hsp90 and CSE forming a multimolecular complex in aortic tissue of rats challenged with T or vehicle (V). Stimulated tissues were homogenized in modified RIPA buffer and 800 μg of total extracts were immunoprecipitated with anti- NR3C4 (AR), anti-hsp90 or anti-CSE antibodies as described in the Methods section. Samples were separated by SDS-PAGE, transferred onto a PVDF membrane and immunoblotted with anti- NR3C4, anti-hsp90 or anti-CSE, as indicated. Representative blots for NR3C4/hsp90 (A), NR3C4/CSE (B) and NR3C4/hsp90/CSE (C) interaction are shown. Statistical analysis was by Student's t-test (*P < 0.05 vs. V; n = 3). No Ab, total cellular extracts incubated with A/G plus agarose beads without antibody; IP, immunoprecipitation with the corresponding antibodies. The experiments were independently performed five times with similar results (n = 5).