Human colonic epithelial cell line, DLD-1 |
CM |
250–400 p.p.m. CO |
↓ iNOS mRNA expression |
1 |
Rat small intestinal epithelial cell line, IEC-6 |
CM |
250 p.p.m. CO |
↓ iNOS protein expression |
2 |
↓ Nitrite production |
TNF-α plus actinomycin D |
250 p.p.m. CO |
↓ Cell death (apoptosis) |
|
|
LPS/hypoxia |
250 p.p.m. CO |
↓ Cell death |
|
|
↓ iNOS protein expression |
|
|
↓ Transcriptional activation of iNOS promoter |
|
Human colonic epithelial cell line, HT-29 |
TNF-α |
CORM-2 (5–20 μM) |
↓ IL-8 mRNA expression |
3 |
↓ ICAM-1 and COX-2 protein expression |
Human colonic epithelial cell line, Caco-2 |
CM |
CORM-2 (50–150 μM) |
↓ Nitrite production |
4 |
↓ iNOS mRNA expression |
↓ IL-8 protein expression |
↓ Transcriptional activation of NF-κB |
↓ IκBα phosphorylation |
↓ MAPK (p38, ERK 1/2 and JNK 1/2) phosphorylation |
Young adult mouse colonic epithelial cell line, YAMC |
No trigger*
|
CORM-2 (1–10 μM) |
↑ Wound repair of cell monolayer |
5 |
Young adult mouse colonic epithelial cell line, YAMC |
TNF-α |
CORM-2 (10 μM) |
↓ KC mRNA expression |
6 |
↓ KC production |
↓ NF-κB activity |
Mouse duodenal epithelial cell line, MODE-K |
TNF-α plus cycloheximide |
CORM-A1 (100 μM) |
↓ Cell death (apoptosis) |
7 |
↑ GSH levels |
↓ ROS production |
↓ Caspase-3/7 activity |