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Flowchart of study design. Samples from 257 patients including 137 patients at first demyelinating event and 120 RRMS patients were subjected to gene expression microarray analysis and randomly divided into a training set (n = 111) and test set (147). training set was used for diagnostic classifier generation and then classifier performance was validated on independent test set. Resampling (n = 43) was done to demonstrate classifier consistency.
