Table 1.
Summary of studies using transcription factor-mediated reprogramming to derive primitive blood progenitors
| Szabo et al (2010) | Pulecio et al (2014) | Pereira et al (2013) | Batta et al (2014) | Doulatov et al (2013) | Riddell et al (2014) | Sandler et al (2014) | |
|---|---|---|---|---|---|---|---|
| Species | Human | Human | Mouse | Mouse | Human | Mouse | Human |
| Starting cell type | Fibroblast | Fibroblast | Fibroblast | Fibroblast | ES cell-derived myeloid restricted progenitor | B-cell progenitor, myeloid progenitor, bone marrow myeloid effector | Umbilical vein endothelial cells, microvascular endothelial cells |
| Transcription factors | OCT4 | SOX2, mir125b | Gata2, Gfi1b, cFos, Etv6 | Erg, Gata2, Runx1c, Scl, Lmo2 | ERG, HOXA9, RORA, SOX4, MYB | Runx1t1, Hlf, Lmo2, Pbx1, Zfp37, Prdm5, Mycn, Meis1 | FOSB, GFI1, RUNX1, SPI1 |
| Factor inducibility | Constitutive | Constitutive | Inducible | Constitutive | Inducible | Inducible | Constitutive |
| Medium | In vitro | In vitro | In vitro | In vitro | In vitro | In vivo | In vitro with endothelial stroma |
| In vitro colony formation | + | + | + | + | + | + | + |
| Erythroid | + | + | Not shown | + | + | + | + |
| Myeloid | + | + | + | + | + | + | + |
| B | − | − | − | −a | − | + | + |
| T | − | − | − | −a | +b | + | +c |
| Engraftment | +d | +d | − | +d | + | + | + |
| Serial Transplantation | − | − | − | − | − | + | + |
| HSC? | No | No | No | No | No | Yes | Nof |
a
Lymphoid differentiation potential acquired with p53 deletion.
b
Although modest T-cell differentiation potential was confirmed in vitro, no T cells were detected in vivo.
c
Minimal in vitro T-cell differentiation possible when TFs are expressed using inducible system.
d
Engrafted cells express low levels of CD45, a pan-lympho-myeloid hematopoietic marker.
e
Very short-term (2 week), primarily erythroid engraftment.
f
In vivo function not assayed with cells derived using inducible system.