Figure 8.

MMS treatment leads to formation of Btn2-dependent non-canonical DNA stress foci at the INQ

1. S. cerevisiae wt cells were treated with MMS and Btn2 levels were determined at the indicated time points. Zwf1 levels are given as a loading control.
2. S. cerevisiae wt and btn2Δ cells expressing Hos2-GFP (green, B) or GFP-VHL (green, C) were grown at 30°C and treated with MMS. Changes in protein localizations were recorded at the indicated time points. DNA was stained by DAPI (blue). The total number of GFP-VHL foci per cell and frequencies (%) of INQ formation were determined.