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LTx and antibody characterization. LTx for standard material was analyzed by non-denaturing gel electrophoresis. The gel was scanned, LF band intensities were quantified, and the amount of free LF was estimated from the linear curve (a). Specificity of anti-LF and anti-PA for LF, PA83, and PA63 alone (b), PA63/LF (LTx) and PA83/LF (c), and LTx standards material; A 100 ng LTx, B 50 ng, C 25 ng, and D 12.5 ng in plasma (d) was assessed by magnetic immunoprecipitation and SDS-PAGE
