Table 5.
Comparison of LTx quantification with other anthrax diagnostic biomarkers, bacteremia (bact), pagA PCR, and total LF, over the course of inhalation anthrax in two rhesus macaques
| Time PC | 16N | 89N | ||||
|---|---|---|---|---|---|---|
| Bact/PCR | LTx | Total LF | Bact/PCR | LTx | Total LF | |
| −30 days | – | <LOD | <LOD | – | <LOD | <LOD |
| 12 h | – | NT | <LOD | – | NT | <LOD |
| 18 h | – | NT | 0.026 | – | NT | <LOD |
| 24 h | – | <LOD | 0.13 ± 0.016 | – | NT | 0.049 ± 0.012 |
| 30 h | – | <LOD | 0.69 ± 0.044 | – | <LOD | 0.32 ± 0.061 |
| 36 h | ±/+ | 0.24 ± 0.041 | 1.8 ± 0.16 | +/+ | 0.10 ± 0.012 | 2.2 ± 0.51 |
| 48 h | +/+ | 0.76 ± 0.074 | 13 ± 1.0 | +/+ | 1.02 ± 0.039 | 30 ± 1.5 |
| 72 h | +/+ | 21 ± 0.54 | 53 ± 3.7 | +/+ | 1.25 ± 0.079 | 20 ± 0.64 |
| 96 h | NA | 5.4 ± 1.4 | 22 ± 0.35 | NA | 12 ± 1.5 | 72 ± 2.7 |
| 120 h | +/+ | 12,100 ± 1270 | 12,200 ± 670 | +/+ | 1150 ± 170 | 1930 ± 44 |
Bact is positive (+) for ≥6 colonies in the primary streak, low positive (±) for one to five colonies, negative (−) for no colonies. Qualitative pagA PCR is positive (+) or negative (−). LOD = 0.0075 ng/mL for LTx and 0.005 ng/mL for total LF. All samples with available volumes were analyzed in duplicate at minimum and at one or more dilutions
PC post-challenge, NA not analyzed