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. Author manuscript; available in PMC: 2016 Apr 16.
Published in final edited form as: Neuroscience. 2015 Feb 17;291:128–145. doi: 10.1016/j.neuroscience.2015.02.012

Figure 3. Repeated stress decreases spine number of LAT neurons in adolescent rats.

Figure 3

A) Examples of Golgi stain in secondary dendrites of LAT neuron from control and stress group (100x magnification). B) Repeated stress decreased the average spine number of principal neurons of the adolescent LAT (box plot with 5-95th percentile, * indicates p<0.05 in two-tailed t-test). C) Overall, there were fewer LAT neurons with higher average spine number after repeated stress than control, demonstrated by a shift in the cumulative frequency probability. D) There was a significant difference in the spine number at 20-60 μm from the soma (* indicates p<0.05 in post-hoc Holms-Sidak's tests). E) There was significantly fewer spines associated with the 4th branch order after stress (* indicates p<0.05 in post-hoc Holms-Sidak's tests). F) When normalized by peak spine number, there was a significant shift in the distribution of spine number across the entire dendritic tree towards 3rd order branches after stress. G) The branch that accounted for the most number of spines shifted from the 4th order to 3rd order after repeated stress (peak order is encircled with gray).