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. Author manuscript; available in PMC: 2016 Mar 19.
Published in final edited form as: Chem Biol. 2015 Mar 5;22(3):379–390. doi: 10.1016/j.chembiol.2015.01.005

Figure 5. Dual inhibition on HIV-1 infection mediated by aptamer-based siRNA delivery system.

Figure 5

A) HIV-1 protection assay. Human PBMC-CD4+ cells were pre-treated with experimental RNAs and then infected with JR-FL virus. B) The siRNA delivered by aptamers knocked down TNPO3 gene expression in human PBMCs. Relative TNPO3 mRNA expression were detected by real-time PCR, with GAPDH as internal control. Asterisk indicates a significant difference compared with control (P < 0.01, student’s t-test). C) HIV-1 challenge assay. Human PBMC-CD4+ cells were infected with JR-FL and then incubated with experimental RNAs. A gp120 aptamer (A-1-stick) and an unrelated aptamer (R-1-stick) were used as positive and negative controls, respectively. Data represent the average of triplicate measurements of p24.