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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Immunol. 2015 Feb 20;194(7):3191–3200. doi: 10.4049/jimmunol.1402443

Figure 7. Notch signaling represses Ccr9 transcription.

Figure 7

Chromatin immunoprecipitation was performed using antibodies against (A) RNA Polymerase II (PolII), (B) AcH3, (C) H3K4me3, and (D) H3K36me3 on extracts from 531026 cells isolated 48 hours after treatment with DMSO (grey) or GSI (black). The precipitated DNA was amplified by QPCR using primers located near the Ccr9 promoter (primers P1 and P2) or within the Ccr9 exons 1 (EX1), 2 (EX2) and 3 (EX3). Primers in a region −88.5 kb upstream of Ccr9 and at the Ebf1 or Deltex1 genes served as controls. Primers at the Deltex1 promoter were used for H3K4me3 and AcH3 and to Deltex1 exon 7 were used for H3K36me3. The data are expressed as enrichment normalized to input and are averaged from three independent experiments. * = p<0.05, ** = p<0.01.