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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Immunol. 2015 Feb 27;194(7):2993–2997. doi: 10.4049/jimmunol.1403086

Figure 2. High Nur77-GFP-expressing GC B cells exhibit SHM.

Figure 2

(A–C) C57/Bl6 reporter splenocytes were stained d14 after NP-KLH immunization. (A) Plots represent gating to identify B220+IgDnegFashiGL7hi GC B cells (left hand panel), and NP-binding GC B cells (middle panels). Overlayed histograms (right hand panel) represent GFP expression from IgD+ (shaded histogram) and GC B cells (blue line). (B,C) Plots and histograms as described in Fig. 1E, F. Data are representative of 8 biological replicates.

(D) Vh186.2 heavy chains isolated from singly sorted GC B cells (see Fig. S1B for gating) from B6 reporter mice d10 after NP-KLH immunization were sequenced. Graphs represent number of mutations in individual clones (see Fig. S1C for mutation distribution). Numbers above graphs show proportion of clones with the W33L mutation.