Skip to main content
. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Immunol. 2015 Feb 20;194(7):3487–3500. doi: 10.4049/jimmunol.1401717

Fig. 3. SIG35α predominantly pairs with TRBV27 TCRβ chains to recognize A2/MART1.

Fig. 3

(A), SIG35α/ΔNGFR-transduced peripheral CD8+ T cells from two HLA-A2+ and two A2− donors were stained with A2/MART1 multimer, mAbs for TCR Vβ subtypes, and anti-CD8 mAb. The percentage of A2/MART1 multimer+ CD8+ T cells expressing each subtype is shown. Data shown are gated on ΔNGFR+ cells. (B), A significant proportion of TRBV27 TCRβ chains in the periphery can recognize A2/MART1 when paired with SIG35α. The percentage of A2/MART1 multimer+ cells in CD8+ TRBV27+ T cells transduced with SIG35α/ΔNGFR gene is shown.

HHS Vulnerability Disclosure