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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Immunol. 2015 Feb 20;194(7):3487–3500. doi: 10.4049/jimmunol.1401717

Fig. 4. TRBV27 TCRβ chains which recognize A2/MART1 when paired with SIG35α are highly heterogeneous and unique.

Fig. 4

SIG35α/ΔNGFR-transduced CD8+ T cells from the HLA-A2+ donor #1 and the A2− donor #3 were stimulated with wt-aAPC or mut-aAPC pulsed with wild-type A2/MART1 peptide. A2/MART1 multimer+ CD8+ T cells were collected by fluorescence activated cell sorting (>99% purity) and their TRBV27 CDR3β regions were amplified by PCR and sequenced after cloning. The number of unique CDR3β sequences (top), the relative usage of Jβ gene segments (middle), and the CDR3β amino acid lengths (bottom) are depicted separately for the A2+ donor #1 (left) and A2− donor #3 (right). Data were analyzed by the aAPC used for stimulations, wt-aAPC vs. mut-aAPC, in each donor.

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