Table IV.

Recognition of MART1–related peptides by Jurkat 76/CD8αβ reconstituted with A2/MART1 TCRs

			TRBV27 chain
No.	Protein	Peptide sequence	Cl.413	Cl.523	Cl.788	Cl.1086	Cl.758	Cl.1593	Cl.1574	Cl.1606	Cl.1599	Cl.830	Cl.794	DMF5
1	KIAA0935	RVTDEAGHPV	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10
2	cMOAT2	NVADIGLHDV	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10
3	SLC1A1	VLTGLAIHSI	<10	<10	<10	<10	<10	<10	29	42	<10	<10	<10	<10
4	P47	RISDIRLFIV	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10
5	Prostaglandin transporter	LLAGIGTVPI	100	248	153	100	205	272	296	277	241	189	283	174
6	ABC transporter MOAT-C	RISDIGLADL	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10
7	KIAA0735	LISGIGIGGA	<10	33	<10	<10	<10	32	100	111	31	23	36	<10
8	Hypothetical 20 kD protein	RISAIILHPN	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10
9	Endothelin-1 receptor	RVQGIGIPLV	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10
10	G-protein coupled receptor RE2	RITDLGLSPH	<10	64	35	<10	124	43	215	213	168	17	248	80
11	IGHG1	RLSELAIFGV	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10
12	Monocarboxylate transporter 8	AVAFIGLHTS	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10
13	MRP3	NVADIGFHDV	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10	<10
14	Wild type MART1	AAGIGILTV	20	102	100	163	198	270	235	216	218	112	272	144

Peptide recognition was assessed using T2 cells loaded with 10 μg/ml of the indicated peptide as stimulator cells in IL-2 ELISPOT assays. Thirteen MART1-related peptides were reported by Dutoit et al. (53). Fifty thousand Jurkat 76/CD8αβ cells, which were individually transduced with eleven distinct TRBV27 TCRβ chains along with SIG35α or with DMF5αβ chains, were used as responder cells. Mean values of SFUs in triplicate samples are shown.