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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Autoimmun Rev. 2015 Jan 13;14(5):415–422. doi: 10.1016/j.autrev.2015.01.003

Figure 1. Secretion of IL-17, IFN-γ, and TNF-α by MCAM+ and MCAM− T cell subsets.

Figure 1

Subpopulations of T cells from a healthy donor were sorted by flow cytometry to separate CD146+ cells from their CD146− counterparts were subsequently cultured for 5 days with CD3/28 stimulation. The cells were then stained for CD146 and three intracellular cytokines, and then analyzed by flow cytometry. Row A: CD4+CD45RO+CD146+ T cells; Row B: CD4+CD45RO+CD146− T cells; Row C: CD8+CD146+ T cells; Row D: CD8+CD146− T cells; Column I: IFN-γ vs IL-17A; Column II: TNF-α vs IL-17A; Column III: IFN-γ vs TNF-α. These data show a marked, but not unique, association of IL-17A with CD146+ T cells. The other two cytokines are abundantly secreted by both CD146+ and CD146− T cells.