Figure 5.

U87-MG and U138 cells were transduced with a Wnt/β-catenin GFP reporter construct. A, reducing CK2α expression using siCK2α decreased β-catenin expression and transcriptional activity (as measured by GFP). B, QPCR analysis of β-catenin-regulated genes, OCT4 and NANOG, in U87-MG cells transfected with siCK2α or siCK2β. C and D, treatment with the CK2 inhibitors, CX-4945 or TBBz, decreased β-catenin expression and transcriptional activity in both U87-MG and U138 cells. E and F, QPCR analysis of OCT4 and NANOG after treatment with CK2 inhibitors. G, β-catenin was stably transduced into U87 shCK2α or U138 shCK2α cell lines. Cells treated were either treated with 5 μg/mL of Dox (+Dox) or a PBS control (−Dox). H, Cell growth of U87 shCK2α cells transduced with β-catenin and treated with Dox. I, Cell growth of U138 shCK2α cells transduced with β-catenin and treated with Dox. F, anchorage-independent growth of U87 shCK2α cells transduced with β-catenin and treated with Dox in soft agar. Results are from two separate experiments, each done in triplicate. *represents a statistically significant change from the control, P < 0.05, as measured by the Mann-Whitney U test