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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Oncogene. 2014 Sep 22;34(29):3770–3779. doi: 10.1038/onc.2014.304

Figure 1. Survivin is expressed in Ptch mutant tumors.

Figure 1

RNA and protein from Ptch mutant MB cells, P7 GNPs and adult cerebellum were analyzed for Survivin expression using real time PCR (A) and by western blotting (B). Survivin is highly expressed in tumors and GNPs, but not in adult cerebellum. Error bars in (A) represent 95% confidence interval calculated using sum of the squares method (p<0.02 by ANOVA and post hoc student's t-test). (C-F) Tissue sections from Ptch mutant tumor and normal adult cerebellum were stained with anti-Survivin antibodies alone (C,E) or with anti-Survivin antibodies that were pre-incubated for 30 min with Survivin blocking peptide (D,F). Survivin is highly expressed in tumor cells with minimal expression in adult cerebellum. Inset in (C) is 4x magnification of positive staining. Scale bars represent 50 μM. Data are representative of 3 experiments.