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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Oncogene. 2014 Sep 22;34(29):3770–3779. doi: 10.1038/onc.2014.304

Figure 2. Loss of Survivin causes decreased proliferation and cell cycle arrest.

Figure 2

(A-B) Cells were isolated from Survivinfl/fl;Ptch+/- tumors and infected with Cre- or GFP retroviruses for 48 hr. (A) GFP+ cells were isolated by flow cytometry and survivin mRNA expression analyzed by RT-qPCR (n=2). Cre causes loss of survivin expression (p<0.02). (B) Cells were pulsed with 3H-thymidine for 12 hr, harvested, and analyzed for incorporation. Loss of survivin leads to decreased tumor cell proliferation (p<0.001). Data are representative of 5 experiments. (C) Cells were isolated from Ptch+/- tumor (wild type for survivin), infected with Cre- or GFP viruses for 48 hr, and collected after 12 hr pulse with 3H-thymidine to measure incorporation. Infection with Cre virus alone does not significantly impair proliferation (p>0.1). Data in (A-C) represent mean +/- standard deviation (SD) and are representative of 4 experiments. (D, E) Cells from Survivinfl/fl;Ptch+/- tumors were infected with virus as described above (D. GFP, E. Cre virus) and stained with 7-AAD for cell cycle analysis by flow cytometry. survivin deletion causes accumulation of cells in G2/M. Data are representative of 4 experiments and cell cycle percentages based on live cell gates (excluded subG1). p values calculated using student's t-test.