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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Mol Carcinog. 2014 Sep 22;54(11):1417–1429. doi: 10.1002/mc.22220

Figure 4.

Figure 4

AsA activates apoptotic machinery in human GBM cells. (A–B) AsA effect on apoptosis and mitochondrial membrane potential (MMP) in GBM cells was analyzed by annexin V/PI and JC1 staining, respectively. (C) GBM cells were treated with AsA for indicated duration and Western blotting was performed. (D–E) LN18 cells were pre-treated for 2 h with pan-caspase inhibitor (CI) Z-VAD.fmk (50 μM) and subsequently incubated with DMSO or AsA (70 μM) for additional 6 h. Thereafter, cells were analyzed for apoptosis by annexin V/PI staining. Under similar treatment conditions, total cell lysates were prepared and immunoblotted for the indicated proteins. Protein loading was monitored by re-probing the membrane with α-tubulin antibody. *, p≤0.001; #, p≤0.01; $, p≤0.05