Role of calcium in the anti-proliferative efficacy of AsA against GBM cells. (A) Effect of AsA treatment on free cytosolic calcium level in GBM cells was examined as described in methods. (B, C & D) GBM cells were pre-treated for 3 h with intracellular calcium chelator BAPTA (5 μM) followed by AsA treatment at indicated dose and time-point. At the end, both adherent and non-adherent cells were collected and live cell number and dead cell percentage were measured by trypan blue exclusion assay. *, p≤0.001; #, p≤0.01; $, p≤0.05