Table 1.
Tumor-initiating capacity of miR-141ZIP compared to SCRZIP T47D cells
|
Number of cells injected per mammary fat
pad |
Number of tumors per number of
injected fat pads a |
|
|---|---|---|
|
| ||
| Week 5 after implantation | ||
| SCRZIP | 141ZIP | |
| 1 × 104 | 2/5 | 5/5 |
| 1 × 103 | 3/10 | 6/10 |
| 1 × 102 | 1/10 | 4/10 |
| Tumor-initiating frequency (95% CI) | (1/7894) | (1/712) |
| Tumor-initiating range (95% CI) | (1/19965 - 1/3121) | (1/1440 - 1/352) |
| P-value | 5.47 × 10−6 | |
| Week 6 after implantation | ||
|---|---|---|
| SCRZIP | 141ZIP | |
| 1 × 104 | 3/5 | 5/5 |
| 1 × 103 | 3/10 | 7/10 |
| 1 × 102 | 2/8b | 5/8b |
| Tumor-initiating frequency (95% CI) | (1/4924) | (1/495) |
| Tumor-initiating range (95% CI) | (1/11721 - 1/2069) | (1/1003 - 1/245) |
| P-value | 3.07 × 10−6 | |
Abbreviation: CI, confidence interval. Limiting dilution analysis of the tumor-initiating frequency of T47D-141ZIP cells as compared with SCRZIP negative control cells.
a
Shown as number of tumors per number of injected fat pads.
b
Two animals were euthanized for health reasons between week 5 and week 6, and were excluded from the analysis. Data were analyzed using ELDA software.