Figure 2. JAK/STAT activation drives overgrowth upon polarity loss.

(A and B) A JAK/STAT pathway reporter (green) is highly elevated throughout dlg as compared to WT discs, indicating strong pathway activation. (C) The ligand-encoding upd genes, but not other JAK/STAT pathway components, are transcriptionally upregulated in neoplastic tissues. (D–G) Reduction of JAK/STAT pathway activity via SOCS36E overexpression has no significant effect on WT growth, but suppresses overgrowth of dlg^hypo tissue. Actin (red) highlights cell outlines, while GFP (green) marks the engrailed-expressing domain. FACS-based quantification is shown in H (**p < 0.001). Scale bars: 100 μm.

DOI: http://dx.doi.org/10.7554/eLife.03189.005

Figure 2—figure supplement 1. upd3 knockdown is not sufficient to prevent neoplastic tumors.

Eye imaginal discs expressing upd3 RNAi alone (A), dlg RNAi alone (B), and dlg RNAi + upd3 RNAi (C). Scale bar: 50 μm.

DOI: http://dx.doi.org/10.7554/eLife.03189.006