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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 21;194(4):1763–1775. doi: 10.4049/jimmunol.1401624

Figure 1. The role of IL-1β NLRP3, ASC and Caspase-1 in S. pneumoniae keratitis.

Figure 1

C57BL/6, IL-1β−/−, Caspase-1/11−/− NLRP3−/− and ASC−/− mice were infected in the corneal stroma with S. pneumoniae TIGR4. A, F, K: Total NIMP-R14+ neutrophils and F4/80+ macrophages in infected corneas were quantified by flow cytometry. B, G, L: Total CFU in eyes of infected mice after 2 h (inoculum) and after 24 h. C, H, M: Representative bright field images of corneas 24h post-infection; D, I, N: Percent, and E, J, O total corneal opacification calculated by Metamorph analysis as shown in Supplemental Figure S1. A, F, K: mean ±SD of 5 samples per group. For CFU and corneal opacification graphs, each data point represents a single cornea. Results are representative of three independent experiments with at least five mice per group.