Figure 2.

Behavioral and molecular responses of Clock^Δ19/Δ19 to restricted feeding. (A) Double-plotted actograms of C57B/6 wild-type (WT) and Clock^Δ19/Δ19 mice under 12L:12D conditions as denoted in the bars at the top. Ad lib food availability is indicated by the gray shading. Step-wise food restriction was carried out from 12 to 8 h (indicated by yellow shading), and the food anticipatory activities (FAA) were detected just prior to food availability (marked by red bars). Two representative actograms are shown for each genotype. (B) Mice were sacrificed on the indicated days of restricted feeding as in (A). Liver samples were collected from three mice per genotype on each of the indicated six time points, every 4 h across the circadian cycle starting from ZT0 (light on). Total RNAs were extracted from these samples and expression of circadian core clock genes are quantified by real-time qPCR analysis. Asterisks indicate the time when circadian phase was reversed by restricted feeding.