Figure 3.
The effects of 20S(OH)D3, 20R(OH)D3, D3, 20S(OH)7DHC, and 20R(OH)7DHC on attenuation of HaCaT cell death induced by UVB. Confluent cultures of HaCaT keratinocytes were irradiated with 0, 25, 50, or 75 mJ/cm2 doses of UVB and then treated with graded concentrations of the steroids and secosteroids. After 48 h, cell viability was measured with the MTS test. Data were analyzed using ANOVA, * p<0.05, ** p<0.01, and *** p<0.001.