Fig 2.

Spermatogenesis regeneration and offspring production after spermatogonial stem cell (SSC) transplantation. (A) The SSCs with membrane fluorescence were settled and incorporated with germ cells of the recipient testis in the injected seminiferous tubules. (B) Red fluorescence was observed in 27% of the pups. (C) Half of the progeny expressed red fluorescence. (D and E) Histological appearance of the recipient testis showing normal spermatogenesis with red membrane fluorescence of spermatogenic cells in some seminiferous tubules. Hoechst indicates nuclear staining.