Fig 3.

DHZ activates AMPK phosphorylation in C2C12 skeletal muscle cells. (A) C2C12 cells were stimulated for 1 hr with various concentrations of DHZ. The cells were then lysed with 2× SDS sample buffer, and the phosphorylation of AMPK was assessed by western blotting using phosphorylation-specific antibody. The level of total AMPK was also assessed as a control for protein loading. The results are representative of four independent experiments. *P < 0.05 versus basal condition. (B) C2C12 cells were treated with 30 μM DHZ for the indicated times. The cells were lysed with 2× SDS sample buffer, and the phosphorylation of AMPK was evaluated by western blotting using phosphorylation-specific antibody. The level of total AMPK was also assessed as a control for protein loading. The results are representative of four independent experiments. *P < 0.05 versus basal condition. (C) C2C12 cells were treated with DHZ and curcumin for the indicated doses. The cells were lysed with 2× SDS sample buffer, and the phosphorylation of AMPK was evaluated by western blotting using phosphorylation-specific antibody.