Fig 4.

Effects of 17-AAG on cell proliferation. (A) 17-AAG diminished cell proliferative activities of LAC cells (A549 and LETPα-2) in a dose-dependent manner compared to the untreated group (each **P < 0.01). (B) The amount of Ki-67 mRNA, indicated by real-time PCR assay, was significantly reduced in a dose-dependent manner in 17-AAG treated groups compared to the untreated group (**P < 0.01). (C) After Lv-Ki-67-siRNA was transfected into LAC cells for 24 hrs, the expression level of Ki-67 mRNA and protein was markedly knocked down. (D) Cell proliferation was significantly repressed in 17-AAG+NC group compared to the 17-AAG+Lv-Ki-67-siRNA, Lv-Ki-67-siRNA and CON groups (**P < 0.01).