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. 2015 Feb 19;13:12. doi: 10.1186/s12915-015-0121-9

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© Garceau et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The effect of chicken CSF1-Fc treatment on bone density. Hatchlings were dosed subcutaneously with 50 μg chicken CSF1-Fc or PBS on hatch day (day 0), day 1, day 2 and day 3, then sacrificed 24 hours after the final injection. Femurs were fixed overnight in 4% paraformaldehyde, and then transferred to 70% ethanol. Micro-computed tomography analysis of the femur was carried out as described in Methods. Error bars represent standard error of the mean. Statistically significant differences were calculated using unpaired t-tests between treatment groups. PBS, phosphate-buffered saline.