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. 2015 Mar 22;7(1):14. doi: 10.1186/s13195-015-0100-y

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© Yang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Oligomer-specific ELISA specifically detects human amyloid-beta species in AD-TBS brain extracts. Soluble extracts of Alzheimer’s disease (AD) brains 1 to 4 (A) and brains 5 to 9 (B) were serially diluted and assayed with the Erenna 1C22/3D6 oligomer-specific enzyme-linked immunosorbent assay (o-ELISA), revealing highly linear concentration curves in the extracts of all brains. SAT, o-ELISA value of the highest loaded standard (50 pg/ml). (C) After pre-clearing with plain beads, AD-TBS extracts of brains 5 to 9 were each subjected to sequential IPs with amyloid-beta antisera 1282 and AW7, and the resultant supernatants assayed by o-ELISA. Western blotting (inset: monoclonal antibodies 6E10 + 2G3 + 21 F12) of a representative immunodepletion experiment shows: lane 1, starting AD extract; lane 2, its supernatant after 1282 + AW7 immunodepletion; and lane 3, IP’ed 1282 + AW7 pellet. TBS, Tris-buffered saline (20 mM Tris–HCl, pH 7.4, containing 150 mM NaCl). Erenna platform (Singulex, Alameda, CA, USA).