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. 2015 Mar 8;13:15. doi: 10.1186/s12958-015-0008-1

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© Margaryan et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Biochemical confirmation of the Hs-8 target specificity. Immunodetection of human sperm extracts and purified recombinant GAPDHS-GST tagged protein with MoAb Hs-8 (A) and with commercial mouse monoclonal antibody against GAPDHS (B). Arrows indicate the GAPDH protein labelled with anti-GAPDHS antibody. St – protein standard, hu sperm – human sperm cell lysate, GAPDHs – recombinant purified GAPDHS protein, hu sperm (SDS) – SDS extract of human sperm cells, hu sperm (RHB) – human sperm cell proteins extracted with RHB buffer.