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. 2015 Mar 8;13:15. doi: 10.1186/s12958-015-0008-1

Figure 5.

Figure 5

Ultrastructural localization of Hs-8 and GAPDHS antigens. Immunogold labelling of the sections of the sperm head (A, B) and principal piece of the tail (C) with Hs-8 (12 nm gold particles, arrowheads) and anti-GAPDHS (6 nm gold particles, arrows) antibodies. The signal after staining with either antibody showed a similar pattern and the gold particles appeared in the fibrous sheath of the principal piece in the tail (C) and acrosomal part of the sperm head (A, B). Bar: 200 nm.