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TUNEL assay across all groups (×400): (a) control group, (b) DOX group, (c) DOX + SMI group, and (d) quantitative analysis of myocardial apoptosis across all groups. The apoptotic nuclei were stained brown, while the normal ones were blue. At the end of follow-up duration, there were 20 survivals in control group, 11 in DOX group, and 16 in DOX + SMI group, respectively. Compared to control group, * P < 0.05; compared to DOX group, # P < 0.05.
