Table 5.
Accuracy and precision for determination of estrogens as NMPS derivatives in serum (n=5).
| Analyte | LQC (1.5 pg/mL) | MQC (75 pg/mL) | HQC (175 pg/mL) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | CV | Accuracy | Mean | SD | CV | Accuracy | Mean | SD | CV | Accuracy | |
| Intra-day | ||||||||||||
| E2 | 1.5 | 0.2 | 10% | 102% | 80.7 | 1.7 | 2% | 108% | 183.8 | 3.0 | 2% | 105% |
| 16α-OH-E2 | 1.8 | 0.1 | 5% | 118% | 79.2 | 1.1 | 1% | 106% | 180.2 | 2.7 | 2% | 103% |
| 4-MeO-E2 | 1.6 | 0.2 | 14% | 105% | 77.9 | 1.6 | 2% | 104% | 176.5 | 8.0 | 5% | 101% |
| 2-MeO-E2 | 1.7 | 0.2 | 9% | 112% | 76.0 | 0.7 | 1% | 101% | 179.3 | 2.5 | 1% | 102% |
| 4-OH-E2 | 1.5 | 0.1 | 6% | 102% | 80.2 | 1.3 | 2% | 107% | 180.6 | 1.0 | 1% | 103% |
| 2-OH-E2 | 1.6 | 0.1 | 6% | 107% | 71.5 | 2.4 | 3% | 95% | 171.4 | 5.1 | 3% | 98% |
| Inter-day | ||||||||||||
| E2 | 1.4 | 0.1 | 4% | 91% | 78.5 | 0.9 | 1% | 105% | 173.2 | 9.0 | 5% | 99% |
| 16α-OH-E2 | 1.5 | 0.2 | 11% | 102% | 79.0 | 1.8 | 2% | 105% | 174.1 | 8.1 | 5% | 99% |
| 4-MeO-E2 | 1.6 | 0.2 | 11% | 105% | 83.2 | 3.7 | 4% | 111% | 185.7 | 10.8 | 6% | 106% |
| 2-MeO-E2 | 1.9 | 0.1 | 5% | 124% | 79.0 | 2.2 | 3% | 105% | 173.7 | 7.6 | 4% | 99% |
| 4-OH-E2 | 1.6 | 0.1 | 8% | 104% | 77.5 | 2.4 | 3% | 103% | 172.4 | 7.3 | 4% | 99% |
| 2-OH-E2 | 1.4 | 0.3 | 20% | 95% | 76.3 | 1.5 | 2% | 102% | 166.0 | 8.1 | 5% | 95% |
SD: standard deviation
CV: coefficient of variation
RE: relative error
RE (%) = (found concentration – actual concentration/actual concentration)×100
Accuracy (%) = RE (%) + 100