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Recovery of E2 from E2-3G-17S after hydrolysis, extraction, derivatization, and analysis (n=5).
| E2-3G-17S (pmol/mL) | β- glucuronidase/ arylsulfatase |
Theoretical E2 from hydrolysis (pg/mL) |
Calculated E2 from hydrolysis (pg/mL) |
Yield (%) |
|---|---|---|---|---|
| 0.037 (10 pg/mL E2 equivalent) |
Without | 0.0 | 0.8 | 8.0 |
| With | 10.0 | 10.5 | 105.0 | |
| 0.370 (100 pg/mL E2 equivalent) |
Without | 0.0 | 0.6 | 6.0 |
| With | 100.0 | 96.6 | 96.6 |
