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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: Methods Mol Biol. 2014;1144:265–281. doi: 10.1007/978-1-4939-0428-0_18

Figure 1.

Figure 1

HSV-1 3D reconstructions. Each of these four reconstructions is radially colored to show surface features of the virus. One of the first icosahedral 3D reconstructions of HSV-1 was of the whole infectious virion (A) (1). The pleomorphic layer of tegument proteins that surround the HSV-1 capsid in the virion are readily visualized in the reconstruction. The first subnanometer resolution reconstruction of HSV-1 was of purified B-capsids (B) (2). The first asymmetric reconstruction of HSV-1 was of purified B-capsids (C) [EMD-5259](3). A portion of the capsid shell has been resected from the reconstruction to show the portal complex sitting beneath a unique vertex of the capsid shell. The first sub-tomogram average from cryo-ET of HSV-1 virions revealed a novel organization of protein in the pleomorphic tegument layer that resembled the tail of bacteriophage (D) [EMD-5453] (4).