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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: Nat Neurosci. 2008 Feb 24;11(3):255–261. doi: 10.1038/nn2056

Figure 6.

Figure 6

A single cysteine is necessary for activation of the TRPV1 channel by cysteine-modifying reagents. The mutation C157A renders the TRPV1 channel insensitive to MTSEA. (a) Representative traces obtained as in Figure 1. The gray trace represents leak current obtained from an inside-out patch from HEK293 cells expressing TRPV1-C157A channels. The blue trace was obtained after applying 2 mM MTSEA to the intracellular face of the membrane patch for 2 min (longer applications of up to 8 min also failed to induce current activation), and the black trace represents currents activated by 4 µM capsaicin. (b) Dose response for activation by capsaicin before (filled symbols) and after (empty symbols) exposure of the inside-out patch to MTSEA. Smooth curves represent fits with the Hill equation. KD values before (118 ± 24 nM) and after (133 ± 32 nM) MTSEA for TRPV1-C157A were not statistically different (Student’s t-test, P > 0.5, n = 5). Data are mean ± s.e.m.