Figure 5. Lung organoids possess multiple types of mesenchymal cells.
(A) D65 HLOs have PDGFRα+ (green) VIM+ (white) double-positive myofibroblasts and PDGFRα−/VIM+ fibroblasts. Scale bar represents 50 µm. (B) D65 HLOs also possesses PDGFRα+ (green) SMA+ (white) double-positive myofibroblasts and PDGFRα−/SMA+ smooth muscle and myofibrblasts. Scale bar represents 50 µm. (C) D65 HLOs do not contain any cartilage whereas positive control iPSC derived teratoma had clear Safranin O staining specific to cartilage. Fast green marks the cytoplasm and hematoxylin the nuclei of both tissues. Scale bar represents 100 µm.