Figure 8. RNA sequencing analysis associates HLOs with fetal lung tissue.
6 HLOs (n = 3 D65 HLOs and n = 3 D110 HLOs) were compared to the undifferentiated H9 stem cells (SC) and definitive endoderm (Def End) and publicly available datasets of adult and fetal human lungs (see Supplementary file 1). (A–B) Plot of the first three principle components generated in the principle component (PC) analysis as pairwise 2-dimensional plots (A) or as an aggregate 3-dimensional projection (B), (C) hierarchical clustering, and (D) Spearman's correlation all demonstrate that HLOs are most closely related to the fetal lung.