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. 2015 Jan;52(1):88–95. doi: 10.1165/rcmb.2013-0399OC

Figure 5.

Figure 5.

Production of active TGF-β1 by Ig-E–stimulated human MCs with or without a tryptase or chymase inhibitor and its effect on PAI-1 production from human airway epithelial cells. (A) PCHMCs were stimulated with IgE receptor cross-linking for 24 hours with or without 1-hour pretreatment of soybean trypsin inhibitor (SBTI) (chymase inhibitor) or Antipain (tryptase inhibitor), and the level of active TGF-β1 was measured by ELISA. Data represent means ± SE of three independent experiments performed in duplicate. *P < 0.05 by Student’s t test. (B) The supernatants from LAD2 cells activated with IgE receptor cross-linking for 24 hours were incubated with SBTI or Antipain for 1 hour. The supernatants from IgE control or IgE-stimulated LAD2 cells with/without SBTI or Antipain were added to BEAS-2B and incubated for 16 hours. Data represent means ± SE of three independent experiments performed in duplicate. *P < 0.05 by Student’s t test.