Table 3.

MyD88 but Not TRIF Modulates Expression of IFN-γ in Lung Neutrophils, Particularly Those in the Bronchoalveolar Lavage Fluid^*

Lung Neutrophils	Study Comparing WT and Myd88−/− Mice		Study Comparing WT and Ticam1−/− Mice
	Myd88−/−	WT	Ticam1−/−	WT
Total neutrophils in BALF ×106	1.0 ± 0.1†	3.2 ± 0.4	5.4 ± 0.3†	3.9 ± 0.2
% IFN-γ+ neutrophils in BALF
% high	13 ± 1.6†	27 ± 3.2	26 ± 2.2	24 ± 2.2
% high + moderate	28 ± 3.4†	51 ± 4.6	51 ± 7.6	51 ± 6.4
Total neutrophils in lung digest after BAL ×106	19 ± 2.0	21 ± 4.0	22 ± 1.3	23 ± 3.0
% IFN-γ+ neutrophils in lung tissue after BALF
% high	16 ± 2.6†	30 ± 2.4	29 ± 1.9	22 ± 3.3
% high + moderate	22 ± 4.1‡	36 ± 5.6	56 ± 3.6	50 ± 6.6
MFI of all neutrophils
BALF	5.9 ± 0.9†	16 ± 2.4	11 ± 1.0	11 ± 0.6
Lung digest	5.0 ± 1.0†	14 ± 1.2	11 ± 1.0	9.0 ± 1.1
MFI of IFN-γ+ neutrophils
BALF	30 ± 1.4	33 ± 3.0	24 ± 3.2	23 ± 2.2
Lung digest	39 ± 2.3	48 ± 7.1	23 ± 1.9	20 ± 1.4

Definition of abbreviations: BAL, bronchoalveolar lavage; BALF, bronchoalveolar lavage fluid; MFI, median fluorescence intensity; WT, wild type.

^*The role of MyD88 and TRIF was examined in neutrophils in BALF and lung tissue after lavage. Flow cytometry was performed to detect intracellular IFN-γ levels in neutrophils (Ly6G+) in the BALF and in the lung tissue after lavage of Streptococcus pneumoniae–infected mice. Isotype-matched control antibody was used for assessing the level of background staining. The mutant genotypes were compared with gender- and age-matched WT mice. Data are expressed as the mean ± SEM (n = 5–7).

^†P ≤ 0.05 compared with WT using two-tailed t tests.

^‡P = 0.07 compared with WT (t test).