Table 5.

Toll-Like Receptors 2 and 4 Are Not Required for IFN-γ Expression by Neutrophils in the Airspace and Lung Tissue^*

Lung Neutrophils	Tlr2/4−/−	WT
Total neutrophils in BALF ×106	3.5 ± 0.4	3.0 ± 0.7
% IFN-γ+ neutrophils in BALF
% high	35 ± 10	37 ± 9.2
% high + moderate	50 ± 5.4	54 ± 2.7
Total neutrophils in lung digest after BAL ×106	38 ± 7.4	26 ± 5.2
% IFN-γ+ neutrophils in lung tissue after BALF
% high	35 ± 3.8	44 ± 7.6
% high + moderate	51 ± 7.9	56 ± 10.4
MFI of all neutrophils
BALF	8.7 ± 2.1	9.6 ± 1.5
Lung digest	8.8 ± 2.3	11 ± 3.2
MFI of IFN-γ+ neutrophils
BALF	17 ± 1.9	17 ± 1.9
Lung digest	18 ± 3.2	17 ± 2.9

Definition of abbreviations: BAL, bronchoalveolar lavage; BALF, bronchoalveolar lavage fluid; MFI, median fluorescence intensity; TLR, Toll-like receptor; WT, wild type.

^*IFN-γ expression was determined in neutrophils (Ly6G+) in the BALF and in the lung tissue after lavage of Streptococcus pneumoniae–infected WT mice and mice lacking both TLR2 and TLR4 (Tlr2/4^−/−). No significant differences were found using t test to compare genotypes (n = 4 from two separate experiments).