Figure 4.

O₃-induced IL-8 in cultures compared with bronchoalveolar lavage (BAL) from healthy volunteers exposed to O₃ in vivo. Concentrations of IL-8 were measured in apical washes from (A) Macs alone, 16HBE cocultures, and 16HBE alone and (B) A549 cocultures and A549 alone collected 1 and 24 hours after exposure to 0.4 ppm O₃ or air for 4 hours; n = 10–11 Macs alone, n = 16–17 16HBE cocultures, n = 15 16HBE alone, n = 6 A549 cocultures, and n = 5 A549 alone. (C) IL-8 was measured in BAL fluid from healthy volunteers exposed to 0.3 ppm O₃ or air for 2 hours in vivo. BAL fluid was collected at 1 and 24 hours after exposure; n = 9–11. *P < 0.05, **P < 0.01, ***P < 0.001, different from air, paired t test; ^#P < 0.05, different from Macs alone, paired t test; ^†P < 0.05, different from A549 alone, paired t test. Data shown are mean ± SEM.