Figure 5.

O₃ exposures increased hyaluronic acid (HA) in cocultures and BAL from healthy volunteers exposed in vivo. Concentrations of HA were measured in apical washes from (A) Macs alone, 16HBE cocultures, and 16HBE alone and (B) A549 cocultures and A549 alone collected 1 and 24 hours after exposure to 0.4 ppm O₃ or air for 4 hours; n = 7 Macs alone, n = 9–10 16HBE cocultures, n = 8 16HBE alone, n = 6 A549 cocultures, and n = 5 A549 alone. (C) HA was measured in BAL fluid from healthy volunteers exposed to 0.3 ppm O₃ or air for 2 hours in vivo. BAL fluid was collected at 1 and 24 hours after exposure; n = 9–11. P values are as indicated or **P < 0.01, different from air, paired t test. Data shown are mean ± SEM.