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. 2015 Mar;52(3):323–331. doi: 10.1165/rcmb.2014-0100OC

Figure 3.

Figure 3.

Influence of LPS stimulation on major histocompatibility complex (MHC) II expression in peritoneal macrophages, MHC II transactivator (CITTA) expression in lung, and lung monocyte M1/M2 phenotype. (A) MHC class II expression (IA-IE PerCPCy55-A) from macrophages in cell media isolated from the peritoneal space of neonatal and juvenile mice treated with thioglycollate. (B) MHC class II expression in macrophages after LPS. Juvenile, but not neonatal, macrophages had a marked increase in MHC class II expression. (C) Lung CITTA levels in juvenile and neonatal mice four days after LPS (5 mg/kg) or saline aspiration. Juvenile, but not neonatal, mice had significantly increased lung expression of CITTA after LPS aspiration above age-matched saline controls (*P < 0.002, n = 5–7 in each group). (D) Neonatal saline-treated mice had significantly fewer lung M2 monocytes compared with neonatal LPS-treated mice (*P < 0.02), neonatal LPS-treated mice had significantly more lung M2 monocytes than M1 monocytes (**P < 0.001) and juvenile LPS-treated mice had significantly more lung M1 monocytes than neonatal LPS-treated mice (***P < 0.04). Error bars represent mean ± SEM. Juv, juvenile; Neo, neonatal.