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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: Stem Cells. 2010 Mar 31;28(3):620–632. doi: 10.1002/stem.301

Figure 5.

Figure 5

Prevention of allo-HSCT-associated GVHD by ES-MDSCs. (A): Survival curve of recipient mice. Lethally irradiated (8 Gy, TBI) BALB/c mice were left untreated (ν, n = 6) or transplanted via tail vein injection with 129SvEv T-cell-depleted bone marrow cells (TCDBM, 5 × 106/mouse) alone (v, n = 6), or TCDBM plus purified 129SvEv splenic T-cells (T, 5 × 105/mouse) (σ, n = 10), or TCDBM plus T and ES-CD115− (2 × 106/mouse) (î, n = 4), or TCDBM plus T and ES-MDSCs (2 × 106/mouse) (λ, n = 11). Two additional treatments of ES-MDSCs (2 × 106/mouse, each) were given to ES-MDSC recipients on days 4 and 10 after the initial transplantation. Data shown are combined results from two independent experiments. (B): Changes of mean body weight in treated mice (values are mean ± SD. v, n = 6; σ, n= 3–10; λ, n= 9–11 mice). (C): Sections of livers stained with hematoxylin and eosin and harvested on day 23 from indicated groups. Representative micrographs are shown. (D): Kinetics of chimerism in the recipient mice. Spleens (SP), livers, and lymph nodes (LN) were recovered from the recipients (n = 3) on days 4, 7, and 14 after transplantation. Donor-derived T-cells (H-2Kb+) were identified by flow cytometry. Representative dot plots of peripheral T-cell chimerism from one of reproducible experiments are presented. The overall allogeneic chimerism was not apparently affected but the frequencies of donor T-cell subsets were increased in the recipients treated with MDSCs. Abbreviations: BM, bone marrow; ES, embryonic stem; LN, lymph nodes; MDSC myeloid-derived suppressor cells; SP, spleens; T, T cells; TCDBM, T-cell-depleted bone marrow.