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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: Stem Cells. 2010 Mar 31;28(3):620–632. doi: 10.1002/stem.301

Figure 6.

Figure 6

Migration pattern of MDSC in GVHD. Irradiated BALB/c mice were injected, via tail vein, with 5 × 106 TCDBM cells and 0.5 × 106 donor T-cells with (n = 5) or without (baseline, n = 4) 1 × 107 PKH26 labeled MDSCs. Mice were killed on day 3 after transfer. Single cell suspensions were prepared from spleen (SP), bone marrow (BM), lymph nodes (LN), liver, and lung and stained with anti-Ly-6C-FITC or isotype control followed by flow cytometric analysis. (A): Absolute numbers of transferred MDSCs in various organs. Significantly higher numbers of transferred MDSCs were found in the spleen (p = .01) and liver (p = .0016). (B): Fold changes of transferred MDSCs in various organs when compared with the baseline (without MDSC transfer). Abbreviations: BM, bone marrow; CT, control; LN, lymph nodes; SP, spleen.