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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: Am J Respir Cell Mol Biol. 2001 Dec;25(6):676–684. doi: 10.1165/ajrcmb.25.6.4321

Figure 1.

Figure 1

Extracellularly supplemented GSH inhibits ceramide generation in A549 cells. Autoradiography of ceramide in lipid extracts of A549 cells treated in the presence of regular medium supplemented with 1% serum with (A) increasing concentrations of GSH (0 to 5 mM) for 3 h or (B) 5 mM GSH for different incubation times. Incubations were terminated by washes with ice-cold PBS, and the cells were harvested with 0.05% trypsin-EDTA. Cellular lipids were extracted and assayed for ceramide by the DAG kinase assay, as described previously (5, 6). The reaction products were analyzed by TLC and autoradiography.