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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: FASEB J. 2007 Oct 30;22(3):910–917. doi: 10.1096/fj.06-7729com

Figure 3.

Figure 3

Cigarette smoke activation of the EGFR is ligand-independent. Serum-starved HAE cells were incubated in DMEM with and without 40 nM 225 mAb on ice for 1 h followed by the indicated treatments in the absence or presence of 225 mAb at 37°C. NT, no treatment; EGF, 100 ng/ml for 15 min; GO, 1 U/ml for 30 min; CS, smoke from 1 cigarette for 45 min. EGFR was immunoprecipitated (IP) with anti-EGFR mAb 225; precipitated proteins were separated by SDS-PAGE, transferred to a nitrocellulose membrane, and immunoblotted (IB) with anti-phospho-Tyr (PY20) and anti-EGFR (RK2) Abs. p-EGFR, phospho-EGFR.